Isolation and Characterization of Phosphorus Solubilizing Bacteria (Burkholderia sp.) from Chinese Soil Rhizosphere
Phosphorus solubilizing bacterium (PSB) was isolated from the rhizosphere of grass area in Yangzhou University, Jiangsu province, China. The aims of this study were to isolate PSB, screen, identify, and assess their effect on the growth of tomato plant. The isolation was done on tricalcium phosphate medium (TCP) which showed good efficient as phosphorus solubilization in both agar plate and liquid assays were obtained on in vitro experiment. The pH of the medium was decreased substantially from 9 to 6.9 in the second week; with enhancing on soluble phosphorus belong to different levels of pH. The inoculation significantly enhanced plant height, stem diameter, leaf area and fresh and dry weight on the tomato plant in pots experiment. The bacterial isolate was identified to be the member of Burkholderia sp. by 16S rDNA gene sequence analysis. A strong positive correlation was found between inoculated soil and uninoculated soil with (PSB) on plant growth and soil available phosphorus.